cd8 fitc  (Miltenyi Biotec)

Journal: Frontiers in Immunology

Article Title: Defined metabolic states shape T cell fate and function across culture conditions

doi: 10.3389/fimmu.2025.1703095

Figure Lengend Snippet: Functional responses of T cells activated in different media conditions. (A–C) Functional assessments were performed on Day 10 post-activation using T cells expanded under representative media and activation conditions. (A) Memory phenotype distribution of CD4 + and CD8 + T cell subsets assessed by flow cytometry using CD45RA and CD62L expression. Box plots represent Tukey distribution. (B) Specific lysis (%) of HiBiT Ramos target cells co-cultured with T cells and blinatumomab at seven effector-to-target (E:T) ratios. Lysis was calculated relative to spontaneous release (Ramos cells alone, no T cells) and maximum lysis (digitonin-treated Ramos). (C) Quantification of cytotoxic activity based on area under the curve (AUC) from E:T response curves. Data summarize two donors. Statistical comparisons were performed using one-way ANOVA with Tukey’s multiple comparisons test. Significance levels are indicated as follows: ns=not significant, p < 0.05 (*), p < 0.01 (**), and p < 0.0001 (****). TM, TexMACS; TA, TransAct; SC, StemCell ImmunoCult.

Article Snippet: CD4 (APC, Miltenyi, Cat. #130-113-210), CD8 (FITC, Miltenyi, Cat. #130-113-875), CD45RA (PE/Cy7, BioLegend, Cat. #304112), and CD62L (BV785, BioLegend, Cat. #304830) were assessed on Day 10.

Techniques: Functional Assay, Activation Assay, Flow Cytometry, Expressing, Lysis, Cell Culture, Activity Assay

Journal: Microorganisms

Article Title: Baculovirus-Displayed ASFV Epitope-Composite Protein Elicits Potent Immune Responses

doi: 10.3390/microorganisms13112468

Figure Lengend Snippet: Overview of the process and application of recombinant baculoviruses generated using the Bac-to-Bac Baculovirus Expression System. The constructed recombinant baculovirus vector, pFastBacDual-GP64SP-FC-VSVG, is illustrated. The target proteins were cloned into a baculovirus vector containing a fusion gene fragment comprising the gp64 SP signal peptide, porcine-derived IgG Fc fragment (pFc), vesicular stomatitis virus G protein (VSV-G), and human cytomegalovirus (CMV) enhancer/promoter cassette. Notably, Bac-RP induces elevated cytokine secretion, IgG titers, and CD4+ and CD8+ T cell activation while maintaining high biosafety in murine models.

Article Snippet: Cells were then stained with PE-labeled CD3, APC-A700-labeled CD4, and FITC-labeled CD8 (Proteintech, Wuhan, China) and incubated at 4 °C in the dark for 30 min. After two PBS washes, fluorescence analysis was performed using flow cytometry (Becton Dickinson FACS Calibur system, Franklin Lakes, NJ, USA) to assess CD3, CD4, and CD8 expression.

Techniques: Recombinant, Generated, Expressing, Construct, Plasmid Preparation, Clone Assay, Derivative Assay, Virus, Activation Assay

Journal: Microorganisms

Article Title: Baculovirus-Displayed ASFV Epitope-Composite Protein Elicits Potent Immune Responses

doi: 10.3390/microorganisms13112468

Figure Lengend Snippet: T cell responses induced by Bac-RP and Bac-p30. BALB/c mice ( n = 3/group) were sacrificed on day 35 post-immunization, and splenic lymphocytes were isolated. ( A ) Quantification of CD8+ and CD4+ T cells by FACS. ( B , C ) Percentage of CD4+ and CD8+ T cells in splenic lymphocytes. Results are expressed as means ± SEM ( n = 3). ***, p < 0.001.

Article Snippet: Cells were then stained with PE-labeled CD3, APC-A700-labeled CD4, and FITC-labeled CD8 (Proteintech, Wuhan, China) and incubated at 4 °C in the dark for 30 min. After two PBS washes, fluorescence analysis was performed using flow cytometry (Becton Dickinson FACS Calibur system, Franklin Lakes, NJ, USA) to assess CD3, CD4, and CD8 expression.

Techniques: Isolation